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Structured Review

Fisher Scientific multiscribe reverse transcriptase
Multiscribe Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multiscribe+reverse+transcriptase/m+mlv+reverse+transcriptase/pm41596270-177-37-41
Average 86 stars, based on 1 article reviews
multiscribe reverse transcriptase - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Isolation:

Article Title: Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines.
Article Snippet: .. Total RNA was isolated from cells treated with or without OpA (1 μM) for 6 h, using TRIzol reagent (15596018, Ambion, Fisher Scientific) according to the manufacturer’s protocol. cDNA was generated using 250 ng of RNA with MultiScribe reverse transcriptase (4319983, Fisher Scientific) according to the manufacturer’s protocol. .. The synthesized cDNA was used to perform real-time quantitative PCR using qPCR blue mix (AZ2305, Azura Genomics, Raynham, MA, USA) by Quantstudio5 system (Applied Biosciences, Fisher Scientific).

Generated:

Article Title: Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines.
Article Snippet: .. Total RNA was isolated from cells treated with or without OpA (1 μM) for 6 h, using TRIzol reagent (15596018, Ambion, Fisher Scientific) according to the manufacturer’s protocol. cDNA was generated using 250 ng of RNA with MultiScribe reverse transcriptase (4319983, Fisher Scientific) according to the manufacturer’s protocol. .. The synthesized cDNA was used to perform real-time quantitative PCR using qPCR blue mix (AZ2305, Azura Genomics, Raynham, MA, USA) by Quantstudio5 system (Applied Biosciences, Fisher Scientific).

Reverse Transcription:

Article Title: Gasdermin D Cleavage and Cytokine Release, Indicative of Pyroptotic Cell Death, Induced by Ophiobolin A in Breast Cancer Cell Lines.
Article Snippet: .. Total RNA was isolated from cells treated with or without OpA (1 μM) for 6 h, using TRIzol reagent (15596018, Ambion, Fisher Scientific) according to the manufacturer’s protocol. cDNA was generated using 250 ng of RNA with MultiScribe reverse transcriptase (4319983, Fisher Scientific) according to the manufacturer’s protocol. .. The synthesized cDNA was used to perform real-time quantitative PCR using qPCR blue mix (AZ2305, Azura Genomics, Raynham, MA, USA) by Quantstudio5 system (Applied Biosciences, Fisher Scientific).

Article Title: Activation of planarian TRPA1 by reactive oxygen species reveals a conserved mechanism for nociception
Article Snippet: Total RNA from UNC22, Smed-AP2 and Smed-TRPA1 knockdown planarians was purified using Trizol reagent (Life Technologies). .. First strand cDNA was synthesized using MultiScribe Reverse Transcriptase (Fisher Scientific) from DNAse-treated (TURBO DNAse, Ambion) total RNA. qPCR reactions were performed using the EvaGreen dye (Biotium). ..

Synthesized:

Article Title: Activation of planarian TRPA1 by reactive oxygen species reveals a conserved mechanism for nociception
Article Snippet: Total RNA from UNC22, Smed-AP2 and Smed-TRPA1 knockdown planarians was purified using Trizol reagent (Life Technologies). .. First strand cDNA was synthesized using MultiScribe Reverse Transcriptase (Fisher Scientific) from DNAse-treated (TURBO DNAse, Ambion) total RNA. qPCR reactions were performed using the EvaGreen dye (Biotium). ..

Real-time Polymerase Chain Reaction:

Article Title: Activation of planarian TRPA1 by reactive oxygen species reveals a conserved mechanism for nociception
Article Snippet: Total RNA from UNC22, Smed-AP2 and Smed-TRPA1 knockdown planarians was purified using Trizol reagent (Life Technologies). .. First strand cDNA was synthesized using MultiScribe Reverse Transcriptase (Fisher Scientific) from DNAse-treated (TURBO DNAse, Ambion) total RNA. qPCR reactions were performed using the EvaGreen dye (Biotium). ..



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